11823 Galt Status: ES cell colonies screened / QC no positives
| Gene | Galt |
| Chromosome | 4 |
| Deletion Start | 41,755,631 |
| Deletion End | 41,758,608 |
| Deletion Size | 2,978 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,756,009 | 41,756,028 | TGTCGGCTCATCGCATGAAG |
| TUP | 41,756,032 | 41,756,054 | CCCTGGCAAGGACAAGTGGAGCC |
| TUR | 41,756,131 | 41,756,151 | TGGCTGTGTATTCGGGCTTAC |
| TDF | 41,758,407 | 41,758,430 | CCCATAAGCCCTCCTCAGTATTTC |
| TDP | 41,758,436 | 41,758,460 | CCCAGAACGCCTCGGTACAATTCCA |
| TDR | 41,758,485 | 41,758,507 | GCGGCCTATAAAGGGACAAGAAG |
PCR Primers
| Primer | Sequence |
| SU | CGGTGTGCAGGAGAATCATC |
| SD | TCAAAGTCCGTAGGCAAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 143 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
