11827 1700011F03Rik Status: ES cell colonies screened / QC positives
| Gene | 1700011F03Rik |
| Chromosome | 6 |
| Deletion Start | 70,868,777 |
| Deletion End | 70,864,314 |
| Deletion Size | 4,464 |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,867,216 | 70,867,236 | TCTAGCTCAGTGTCTGGGAAC |
| TUP | 70,867,187 | 70,867,214 | TCTAATCCTCTGCCTGCTATCCTAGGCC |
| TUR | 70,867,140 | 70,867,163 | GCCATGTGAGATTTAGGGTAAGTG |
| TDF | 70,864,087 | 70,864,106 | TGCATGAGGACCTGGGTTTG |
| TDP | 70,864,052 | 70,864,078 | AACCCACAGGCACAGTAGCATGTCTCA |
| TDR | 70,864,024 | 70,864,045 | CCTGTCTGTCTCCCTAGCACTG |
PCR Primers
| Primer | Sequence |
| SU | AACGGATCTGATATTTTGAGTG |
| SD | CTACATACATTCTATACACACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11827A-A1 | VGB6 | C57BL/6NTac | |
| 11827A-A12 | VGB6 | C57BL/6NTac | |
| 11827A-E3 | VGB6 | C57BL/6NTac | |
| 11827A-D3 | VGB6 | C57BL/6NTac | |
| 11827A-C6 | VGB6 | C57BL/6NTac | |
| 11827A-F3 | VGB6 | C57BL/6NTac | |
