11827   1700011F03Rik     Status: ES cell colonies screened / QC positives

Gene1700011F03Rik
Chromosome6
Deletion Start70,864,314
Deletion End70,868,777
Deletion Size4,464
Genome Build37
MGI IDMGI:1921471
NCBI Gene ID74221
BAC ID158P8
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 6

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFTCTAGCTCAGTGTCTGGGAAC
TUPTCTAATCCTCTGCCTGCTATCCTAGGCC
TURGCCATGTGAGATTTAGGGTAAGTG
TDFTGCATGAGGACCTGGGTTTG
TDPAACCCACAGGCACAGTAGCATGTCTCA
TDRCCTGTCTGTCTCCCTAGCACTG

PCR Primers

PrimerSequence
SUAACGGATCTGATATTTTGAGTG
SDCTACATACATTCTATACACACC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTCATTCTCAGTATTGTTTTGCC
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11827A-A1VGB6C57BL/6NTac
11827A-A12VGB6C57BL/6NTac
11827A-E3VGB6C57BL/6NTac
11827A-D3VGB6C57BL/6NTac
11827A-C6VGB6C57BL/6NTac
11827A-F3VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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