| Gene | Gm614 |
| Chromosome | X |
| Deletion Start | 101,263,846 |
| Deletion End | 101,261,383 |
| Deletion Size | 2,464 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,263,609 | 101,263,631 | AAGTGGTTTAGGGAGGAGTAGTG |
| TUP | 101,263,581 | 101,263,607 | CGAAGGTCAGAAGCAATAAGCTGCCTC |
| TUR | 101,263,554 | 101,263,576 | CTTGGTGGGAGGAAAGTTTAGAG |
| TDF | 101,262,102 | 101,262,124 | CCCTACTTCCTTCCCATCATTTC |
| TDP | 101,262,075 | 101,262,098 | CTTGGGCCAAGCTACTCTGACCGA |
| TDR | 101,262,046 | 101,262,068 | TCCATCGTGATCTTCTGTCATTC |
PCR Primers
| Primer | Sequence |
| SU | CAGTGTCTTCTCTCCCTGGC |
| SD | TTTCAGCCTGGGAGGTAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11829A-A5 | VGB6 | C57BL/6NTac | |
| 11829A-D3 | VGB6 | C57BL/6NTac | |
| 11829A-E8 | VGB6 | C57BL/6NTac | |
| 11829A-F7 | VGB6 | C57BL/6NTac | |
