11830 H3f3b Status: Cancelled
| Gene | H3f3b |
| Chromosome | 11 |
| Deletion Start | 116,026,467 |
| Deletion End | 116,025,868 |
| Deletion Size | 600 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,023,791 | 116,023,810 | CGTTGACGCCTTCCTTCTTC |
| TUP | 116,023,768 | 116,023,787 | TGACTGCAGGCCAGGGACCG |
| TUR | 116,023,720 | 116,023,741 | TGAGCAGCTCAGTCGATTTCTG |
| TDF | 116,023,549 | 116,023,570 | AGGAGTGTTAATGCTGTTGCTC |
| TDP | 116,023,525 | 116,023,547 | TGTCCTCAACAGGAGGCTAGCGA |
| TDR | 116,023,501 | 116,023,522 | TCAAACAACCCCACCAGGTATG |
PCR Primers
| Primer | Sequence |
| SU | CGGTGGTGACTCACAGGTC |
| SD | AAGCAAGAAGTATCACCCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
