11831 Dynll1 Status: ES cell colonies screened / QC no positives
| Gene | Dynll1 |
| Chromosome | 5 |
| Deletion Start | 115,300,661 |
| Deletion End | 115,298,716 |
| Deletion Size | 1,946 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,300,411 | 115,300,429 | GGCTCGGGATGGATAGTTC |
| TUP | 115,300,385 | 115,300,410 | TAGAAAGGACGCAAGGGTTTCGCGTT |
| TUR | 115,300,357 | 115,300,376 | GCGTTGAGGGTCTGGTCTTC |
| TDF | 115,299,350 | 115,299,368 | ACAGTGGTGGAGCCTGATG |
| TDP | 115,299,317 | 115,299,346 | TGCAAATGCTGATGTCATCTACCTCCATCG |
| TDR | 115,299,289 | 115,299,308 | CTGCACCTCACCTCTGACTC |
PCR Primers
| Primer | Sequence |
| SU | CTGTCTTCTGCTGCTTGAGC |
| SD | AGAGACATACATCTCCAACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
