11832 Vmo1 Status: Cancelled
| Gene | Vmo1 |
| Chromosome | 11 |
| Deletion Start | 70,514,569 |
| Deletion End | 70,513,533 |
| Deletion Size | 1,037 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,514,418 | 70,514,436 | ACTTTGCCAGTGGGTTCTC |
| TUP | 70,514,390 | 70,514,415 | TCAAGGTGAAGGTTCAGACCTGGGTC |
| TUR | 70,514,333 | 70,514,356 | GAGGATTCTCCTTCTCACGTTCAG |
| TDF | 70,513,816 | 70,513,837 | TGTCCTGGCACTAGCTTCTTAG |
| TDP | 70,513,791 | 70,513,811 | TTTCTGCCTCCGGGTGGAGCC |
| TDR | 70,513,751 | 70,513,772 | CACGTTGTTCACTCCAGTGTTG |
PCR Primers
| Primer | Sequence |
| SU | AACTCAGTGGCTTCTACTGC |
| SD | TGAAGTCACTGATGGTACGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
