11833 Ccng2 Status: Cancelled
| Gene | Ccng2 |
| Chromosome | 5 |
| Deletion Start | 93,268,625 |
| Deletion End | 93,274,997 |
| Deletion Size | 6,373 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 93,269,496 | 93,269,517 | GTAGCTCTGACTGTTGTGGAAC |
| TUP | 93,269,523 | 93,269,547 | CTATAGACTCGGCTGGCCTCCAACC |
| TUR | 93,269,551 | 93,269,569 | GCAGAAGCGAGCAGAACTC |
| TDF | 93,274,370 | 93,274,391 | TGCTCTTGCTTTGGTGTTTGAG |
| TDP | 93,274,394 | 93,274,423 | AACCGGAGAAGGAGTGACTGATACAAAGGA |
| TDR | 93,274,450 | 93,274,470 | GCAGAGGTAACCACAGAAGTC |
PCR Primers
| Primer | Sequence |
| SU | CAGAGCTGATCAGAACTAGC |
| SD | GGAATGAAGGAATCAGCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
