11835 Loh12cr1 Status: ES cell colonies screened / QC no positives
| Gene | Loh12cr1 |
| Chromosome | 6 |
| Deletion Start | 134,641,203 |
| Deletion End | 134,644,468 |
| Deletion Size | 3,266 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,641,430 | 134,641,449 | GGTTCCTGGACTCCACACTG |
| TUP | 134,641,450 | 134,641,474 | ACGTGGAGACTTGGGTCACACTGGA |
| TUR | 134,641,480 | 134,641,501 | GATCTTGGAGGACTCGGGTATG |
| TDF | 134,644,370 | 134,644,388 | ATCGTGGTGGTAGCTCAGG |
| TDP | 134,644,391 | 134,644,414 | TCTCAGGCCTCACGGAATGTCAGC |
| TDR | 134,644,444 | 134,644,462 | GGGCTGGAAGGTTGGAATC |
PCR Primers
| Primer | Sequence |
| SU | GCCTAGCCCTTTAAGGCAG |
| SD | ATTTACCACAGGTCAATGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
