11837 Rccd1 Status: ES cell colonies screened / QC no positives
| Gene | Rccd1 |
| Chromosome | 7 |
| Deletion Start | 80,324,191 |
| Deletion End | 80,320,031 |
| Deletion Size | 4,161 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,320,882 | 80,320,900 | AAGTGAGCGCAGGCTGGAG |
| TUP | 80,320,859 | 80,320,880 | TACACTGCCCTGGTGACCCGTG |
| TUR | 80,320,828 | 80,320,848 | ACAGGTCTGGAGTTGGGATTG |
| TDF | 80,317,098 | 80,317,116 | GGGCTGGCATCTTTCTGAG |
| TDP | 80,317,062 | 80,317,087 | CAAGGCCTAGAAGAGGAAGGGCTTCC |
| TDR | 80,317,013 | 80,317,030 | GCTGGTGCTGTCCTTGTG |
PCR Primers
| Primer | Sequence |
| SU | GTGGGATGGACTCCGTTTG |
| SD | AGCTAAGCTGTTGGTGCACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
