11838 Atp6v0b Status: Cancelled
| Gene | Atp6v0b |
| Chromosome | 4 |
| Deletion Start | 117,887,115 |
| Deletion End | 117,884,431 |
| Deletion Size | 2,685 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 117,886,616 | 117,886,639 | GGAATCTGCTACACCATCTTTGAC |
| TUP | 117,886,592 | 117,886,614 | TGGGCTTTCGCTTTGATGTGGCA |
| TUR | 117,886,532 | 117,886,552 | TCAGACCAAAGCAGGGAGAAG |
| TDF | 117,885,280 | 117,885,302 | TCCTCCAGGTTACTCCATGTTTG |
| TDP | 117,885,254 | 117,885,275 | TGGCCTCACAGTCGGTCTGTCC |
| TDR | 117,885,222 | 117,885,240 | ACGATGCCCACGCAGACTC |
PCR Primers
| Primer | Sequence |
| SU | GATCTGCAGTGATTGGTGAG |
| SD | TCCTTTGTGAAAGGTGTCGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
