| Gene | S100a13 |
| Chromosome | 3 |
| Deletion Start | 90,515,893 |
| Deletion End | 90,524,499 |
| Deletion Size | 8,607 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,515,919 | 90,515,937 | GGCGGCCATTGAGACTGTG |
| TUP | 90,515,945 | 90,515,971 | CTTTCTTCACCTTTGCAGGGCGGGAAG |
| TUR | 90,515,976 | 90,516,000 | TTCATTGATGTTCAGGCTGCCTTTG |
| TDF | 90,524,194 | 90,524,216 | AGTTTACGATCAGCCATAGTGTC |
| TDP | 90,524,220 | 90,524,245 | TCGTGTCCCTCGGATATGAGACCTCC |
| TDR | 90,524,272 | 90,524,290 | TGCAGATGCCTTCCCTCAG |
PCR Primers
| Primer | Sequence |
| SU | TTGTCCCATGGAAGGATCAC |
| SD | CACACATGCTCAAGGACCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11839A-A2 | VGB6 | C57BL/6NTac | |
| 11839A-A4 | VGB6 | C57BL/6NTac | |
| 11839A-D6 | VGB6 | C57BL/6NTac | |
| 11839A-E2 | VGB6 | C57BL/6NTac | |
| 11839A-H4 | VGB6 | C57BL/6NTac | |
| 11839A-D11 | VGB6 | C57BL/6NTac | |
