11841 Aipl1 Status: Cancelled
| Gene | Aipl1 |
| Chromosome | 11 |
| Deletion Start | 72,037,060 |
| Deletion End | 72,028,778 |
| Deletion Size | 8,283 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,036,604 | 72,036,625 | GGTGCGACACCATTGTAAGTAG |
| TUP | 72,036,584 | 72,036,603 | TCTCGTGGCTCCCTCTGCAC |
| TUR | 72,036,565 | 72,036,582 | AAGCGCGTGAGGGAACAG |
| TDF | 72,029,971 | 72,029,993 | GAGGTGTCTGCTTCTGACTGAGG |
| TDP | 72,029,948 | 72,029,970 | CCGGCGATCTAGTCCAGGCTCAC |
| TDR | 72,029,906 | 72,029,926 | ATGGCACTGGAGGCTGTGAGG |
PCR Primers
| Primer | Sequence |
| SU | TCTGCATCTGCTGAGAGATC |
| SD | TGTGATTTAATACGGATATGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 61 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
