11844 Rbmx2 Status: Cancelled
| Gene | Rbmx2 |
| Chromosome | X |
| Deletion Start | 48,695,119 |
| Deletion End | 48,710,101 |
| Deletion Size | 14,983 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,695,314 | 48,695,332 | GCGCAGCCCTCTGACTAAG |
| TUP | 48,695,334 | 48,695,362 | TGAAGCTGATCAATGAACTGAATGAGCGA |
| TUR | 48,695,363 | 48,695,382 | GCCACTCCAAGTTGAACCTC |
| TDF | 48,709,202 | 48,709,224 | GCAGAGGTATCTAAGGGAGGAAG |
| TDP | 48,709,228 | 48,709,252 | AGTCGTGCGGGCAGAATTAATGTGC |
| TDR | 48,709,254 | 48,709,273 | GAGATCCGCAAGGGTTTCAG |
PCR Primers
| Primer | Sequence |
| SU | ACTGGCAGAGGAACACAGG |
| SD | GGTTTGTGGGTTTAAGGGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
