11846   Lage3     Status: ES cell colonies screened / QC no positives

GeneLage3
ChromosomeX
Deletion Start74,352,503
Deletion End74,351,346
Deletion Size1,158
Genome Build38
MGI IDMGI:1913442
NCBI Gene ID66192
BAC ID437K13
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome X

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,353,00674,353,027GGTGCCTTTCCTGAGTCTCTAC
TUP74,352,98274,353,005CAGCTCCCTCTTCGTGCCTTTCCC
TUR74,352,94874,352,965GGACCCACAGGCGATTTC
TDF74,352,83874,352,858GTCTAGGATGCTGGGTAGGTG
TDP74,352,81774,352,837TCTGGGCCATCCTCCACCCTA
TDR74,352,78874,352,811TGTGATGATGTCTCTGAGGAAGTG

PCR Primers

PrimerSequence
SUAACTTGGCATGGTGATGCAC
SDGCTCAAATGCAGATTAGCAAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR80 bp++-
TDFTDR71 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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