| Gene | 5730403B10Rik |
| Chromosome | 16 |
| Deletion Start | 4,789,144 |
| Deletion End | 4,787,558 |
| Deletion Size | 1,587 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,769,559 | 4,769,579 | TGGCAGCTAGGAGGAACTTTG |
| TUP | 4,769,530 | 4,769,555 | CCACCAAGGAAGGTTACAGTGGCTCA |
| TUR | 4,769,503 | 4,769,522 | GCTCCCACCTTACCACAGAC |
| TDF | 4,769,131 | 4,769,148 | GCCTCTTCCTGCTGACAC |
| TDP | 4,769,106 | 4,769,130 | TCTGACCCTCTTCTGGAAGACAGCA |
| TDR | 4,769,075 | 4,769,094 | TCCAACACTCAACGCACAAC |
PCR Primers
| Primer | Sequence |
| SU | TGCTGGATCTATCCACCAAC |
| SD | AGCAACACACAAGATTAGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11854A-A1 | VGB6 | C57BL/6NTac | |
| 11854A-E1 | VGB6 | C57BL/6NTac | |
| 11854A-E7 | VGB6 | C57BL/6NTac | |
| 11854A-H4 | VGB6 | C57BL/6NTac | |
