11855 Fut1 Status: ES cell colonies screened / QC no positives
| Gene | Fut1 |
| Chromosome | 7 |
| Deletion Start | 45,618,627 |
| Deletion End | 45,619,757 |
| Deletion Size | 1,131 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,618,886 | 45,618,908 | GAGGCAGGTTTGGTAACCAGATG |
| TUP | 45,618,915 | 45,618,939 | TATGCTACATTGCTGGCCCTAGCCC |
| TUR | 45,618,944 | 45,618,962 | GAAGGCTTGGCGACCATTG |
| TDF | 45,619,283 | 45,619,302 | TGTGCGTCGTGGAGACTATC |
| TDP | 45,619,309 | 45,619,331 | TGATGCCCAATCGCTGGAAGGGT |
| TDR | 45,619,339 | 45,619,357 | TGGAGGTAAGCTCGGTCAC |
PCR Primers
| Primer | Sequence |
| SU | TTGCCTGGGAGGCAGAATG |
| SD | AGACCAGTTGGTAAGTCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
