11858 Atp5g1 Status: Germline transmission achieved
| Gene | Atp5g1 |
| Chromosome | 11 |
| Deletion Start | 95,930,814 |
| Deletion End | 95,932,767 |
| Deletion Size | 1,954 |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CCCAGGTGATGCCAATGTTC |
| TUP | | | TGCCAAGCCTGAGAGTCATTGTACTGA |
| TUR | | | CCATCTTAGGAAAGCTCCCAGTG |
| TDF | | | TGGTGTGGCTGGATCAGGAG |
| TDP | | | CTGGCATTGGCACAGTGTTTGGTAGC
|
| TDR | | | CCAAGTGGAGTGGAGCAACAAC |
PCR Primers
| Primer | Sequence |
| SU | TCGGTTGCTGTCCTTCTGC |
| SD | AAGTGACAAAGCACCAACATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11858A-D10 | VGB6 | C57BL/6NTac | |
| 11858A-F4 | VGB6 | C57BL/6NTac | |
| 11858A-F10 | VGB6 | C57BL/6NTac | |
| 11858A-F12 | VGB6 | C57BL/6NTac | |
