| Gene | Mllt11 |
| Chromosome | 3 |
| Deletion Start | 95,227,308 |
| Deletion End | 95,226,986 |
| Deletion Size | 323 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,220,421 | 95,220,441 | GTAGCCAGTACAGCTCCTTTC |
| TUP | 95,220,396 | 95,220,418 | TTCTGGAGGATGCCCATCCCAGA |
| TUR | 95,220,337 | 95,220,358 | TGCTCTCGTAGGTAGGTGTATC |
| TDF | 95,220,246 | 95,220,265 | CCCCTCCTTGAGTACAGCAC |
| TDP | 95,220,218 | 95,220,243 | TCAACTTCTGGAGAGCGCCTATTGCC |
| TDR | 95,220,182 | 95,220,202 | GCCTTAAAGCAAGTCCAGGTC |
PCR Primers
| Primer | Sequence |
| SU | TAGTGTGCATAAGGCCTGAG |
| SD | TGGTGCCTCAAATAAGGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11860A-C11 | VGB6 | C57BL/6NTac | |
| 11860A-D2 | VGB6 | C57BL/6NTac | |
| 11860A-E11 | VGB6 | C57BL/6NTac | |
