11861 Alox12b Status: ES cell colonies screened / QC no positives
| Gene | Alox12b |
| Chromosome | 11 |
| Deletion Start | 69,157,250 |
| Deletion End | 69,169,812 |
| Deletion Size | 12,563 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,157,729 | 69,157,747 | CTCAGCCACGTTGCATCAC |
| TUP | 69,157,751 | 69,157,779 | TGGCTTCCATTTGCATTGGATTCTCTGTG |
| TUR | 69,157,815 | 69,157,835 | TCCATTAGGGCAAAGCAAGTC |
| TDF | 69,169,005 | 69,169,026 | GAGTCCCTCTTCAACCTCAGTG |
| TDP | 69,169,028 | 69,169,052 | CCATGGAAACCCTGGTCCAGTCTCA |
| TDR | 69,169,055 | 69,169,073 | GCCTACAGATGGGCGAGAC |
PCR Primers
| Primer | Sequence |
| SU | TCTTCTATCCGCCCTCTCAG |
| SD | TCACCCTGGATCTGACTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
