11862 Crk Status: Cancelled
| Gene | Crk |
| Chromosome | 11 |
| Deletion Start | 75,679,427 |
| Deletion End | 75,703,506 |
| Deletion Size | 24,080 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,679,976 | 75,679,995 | GGGACATCATTTGGCAGTGG |
| TUP | 75,680,000 | 75,680,029 | CAGAATGGCTCTGCTAATCCTACAAGTGGC |
| TUR | 75,680,055 | 75,680,073 | CCAAACCCGGCTCTAACAC |
| TDF | 75,702,823 | 75,702,844 | TGTGGCCTGTACCTTCTCTTAG |
| TDP | 75,702,846 | 75,702,868 | TGCTTGGCCTTTGCTGAGACACA |
| TDR | 75,702,869 | 75,702,886 | GCTGCATTCGGACCCTAC |
PCR Primers
| Primer | Sequence |
| SU | TCAATTTGTAGGTTGAGGCTC |
| SD | CCCAAAAGAAAGGAGGCAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 64 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
