11863 Vpreb1 Status: ES cell colonies screened / QC no positives
| Gene | Vpreb1 |
| Chromosome | 16 |
| Deletion Start | 16,869,247 |
| Deletion End | 16,868,545 |
| Deletion Size | 703 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 16,869,059 | 16,869,079 | GGCCTATCTCACAGGTAAGGA |
| TUP | 16,869,021 | 16,869,047 | CCCAGGGCTTCTTTGCTCCTCCTATGG |
| TUR | 16,868,967 | 16,868,986 | GAGGGCCACAACCTGAGAAG |
| TDF | 16,868,647 | 16,868,667 | CGGAGCCAGGAAAAGAAGAGG |
| TDP | 16,868,611 | 16,868,640 | AGGGAGTGGGAAGGAGAAAAGTCGTATACA |
| TDR | 16,868,537 | 16,868,558 | CAGACTAGCCGAGCAAAGCAAA |
PCR Primers
| Primer | Sequence |
| SU | ACGTTCCAGCAGGTGCTTC |
| SD | GTAACAATGAGGTAAACCTGCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 113 bp | + | + | - |
| TDF | TDR | 131 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
