| Gene | Fus |
| Chromosome | 7 |
| Deletion Start | 127,967,568 |
| Deletion End | 127,981,835 |
| Deletion Size | 14,268 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,968,439 | 127,968,460 | TGAGCTACAGCTTCCCAGATTG |
| TUP | 127,968,461 | 127,968,486 | TTGAAAGTGCTGTCTGCTTTGGGTGC |
| TUR | 127,968,519 | 127,968,537 | CCAAGGTTCCCGACGTGTG |
| TDF | 127,981,299 | 127,981,317 | GGGAGCAGAGCTGGAACTG |
| TDP | 127,981,318 | 127,981,341 | AGAGCTCCAGAAAGAGCCCCACAC |
| TDR | 127,981,352 | 127,981,371 | CTTCCCTCCCGTGACCTTTG |
PCR Primers
| Primer | Sequence |
| SU | GCAAGGAGAAAGCGCGTG |
| SD | TCCAATTCTTGCTGTGCAAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11866A-A7 | VGB6 | C57BL/6NTac | |
| 11866A-B11 | VGB6 | C57BL/6NTac | |
| 11866A-C4 | VGB6 | C57BL/6NTac | |
| 11866A-C7 | VGB6 | C57BL/6NTac | |
| 11866A-D6 | VGB6 | C57BL/6NTac | |
| 11866A-G1 | VGB6 | C57BL/6NTac | |
