11869 Neurog2 Status: ES cell colonies screened / QC no positives
| Gene | Neurog2 |
| Chromosome | 3 |
| Deletion Start | 127,633,732 |
| Deletion End | 127,635,333 |
| Deletion Size | 1,602 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,634,044 | 127,634,063 | GTGCAGCGCATCAAGAAGAC |
| TUP | 127,634,065 | 127,634,086 | CGCAGGCTCAAGGCCAACAACC |
| TUR | 127,634,104 | 127,634,123 | AGCGCGGCGTTTAGGTTGTG |
| TDF | 127,635,015 | 127,635,037 | TGGCATCTGCTCTATTCCCATTG |
| TDP | 127,635,041 | 127,635,064 | TCTGCAGGCTGTGGGAATTTCACC |
| TDR | 127,635,073 | 127,635,095 | GCACATCAGAGAGGGAAAGTTTG |
PCR Primers
| Primer | Sequence |
| SU | TCTCCACTTCGCAGGCAGT |
| SD | TCGGGATGTAAATACATACTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
