| Gene | Praf2 |
| Chromosome | X |
| Deletion Start | 7,728,587 |
| Deletion End | 7,730,416 |
| Deletion Size | 1,830 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,729,097 | 7,729,120 | TCAGCGATATGAGTGGCAGATTAG |
| TUP | 7,729,124 | 7,729,149 | AGCCCTGGAAGGGTTAATCCAGAGGT |
| TUR | 7,729,156 | 7,729,177 | TCCCAAGCCCTGACTTTACTAC |
| TDF | 7,729,537 | 7,729,559 | GTGAGGGTCTGGTAGGATTGTAG |
| TDP | 7,729,562 | 7,729,588 | TCGAAGAGGAACTGAGAGTCCACAGGA |
| TDR | 7,729,619 | 7,729,639 | GGAATTAGCATCCCGGCATAG |
PCR Primers
| Primer | Sequence |
| SU | AAGAGTTTTGAGCTGCTCGC |
| SD | CAAGACTGGAAGTAGGTGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11874A-B8 | VGB6 | C57BL/6NTac | |
| 11874A-C4 | VGB6 | C57BL/6NTac | |
| 11874A-G10 | VGB6 | C57BL/6NTac | |
