11875 Plekha1 Status: Cancelled
| Gene | Plekha1 |
| Chromosome | 7 |
| Deletion Start | 130,877,757 |
| Deletion End | 130,911,203 |
| Deletion Size | 33,447 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 130,878,239 | 130,878,259 | GTCCACTGAGTGGTGTCATTC |
| TUP | 130,878,260 | 130,878,279 | TGTGATGGAAATGGACTTTC |
| TUR | 130,878,286 | 130,878,310 | ATCCCAGAGACTTACAAGGAACAAC |
| TDF | 130,910,804 | 130,910,824 | TGTGTCTAACCACCATTCCTC |
| TDP | 130,910,829 | 130,910,849 | CAGGAGCATTCCGGCTGGCCT |
| TDR | 130,910,851 | 130,910,873 | ACGGACAGTGAATTTGGGTTCTG |
PCR Primers
| Primer | Sequence |
| SU | TGGATCATCTTGATTTGTGAGG |
| SD | TCAAAGGGAGTCATATTAGCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
