11876 Tfap2b Status: Cancelled
| Gene | Tfap2b |
| Chromosome | 1 |
| Deletion Start | 19,209,171 |
| Deletion End | 19,234,415 |
| Deletion Size | 25,245 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,209,352 | 19,209,372 | TGATCGGGTTTCTGAGGTTTC |
| TUP | 19,209,376 | 19,209,403 | AAGTTTTCTAAGGCTGGCTCTGGGAGAC |
| TUR | 19,209,413 | 19,209,437 | TCCAACCTTAAAAGTTCCCTTTCTG |
| TDF | 19,234,237 | 19,234,254 | GCGCTCAAAGGCATGGAC |
| TDP | 19,234,259 | 19,234,288 | TGTTCTTGAACAACACCACTAACAGGCACA |
| TDR | 19,234,321 | 19,234,342 | TCCTGTGTTTCTCCTCCTTGTC |
PCR Primers
| Primer | Sequence |
| SU | TGGATTCTGTGACCCAGAGG |
| SD | AATGAGGAGAAATGGAGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
