| Gene | Ctsk |
| Chromosome | 3 |
| Deletion Start | 95,500,838 |
| Deletion End | 95,508,932 |
| Deletion Size | 8,095 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,500,864 | 95,500,884 | CATGGTGAGCTTTGCTCTGTC |
| TUP | 95,500,885 | 95,500,907 | TCCGGAGGAAATGCTGGACACCC |
| TUR | 95,500,940 | 95,500,961 | TGGGCACCTTGCTGTTATACTG |
| TDF | 95,508,853 | 95,508,872 | TGCTCTCTTGGCTCGGAATA |
| TDP | 95,508,877 | 95,508,899 | CAACGCCTGCGGCATTACCAACA |
| TDR | 95,508,913 | 95,508,933 | GCTGGCTGGAATCACATCTTG |
PCR Primers
| Primer | Sequence |
| SU | GTCTGTGTGTGTATGTGGTG |
| SD | ACAGAGTCCACAACTCTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11877A-F3 | VGB6 | C57BL/6NTac | |
| 11877A-F7 | VGB6 | C57BL/6NTac | |
