11878 Scn1b Status: Cancelled
| Gene | Scn1b |
| Chromosome | 7 |
| Deletion Start | 31,126,331 |
| Deletion End | 31,116,851 |
| Deletion Size | 9,481 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,125,832 | 31,125,851 | GTGGGTGAAAGAGGGACCAG |
| TUP | 31,125,807 | 31,125,831 | AGGCCTCTAACCCAGTGGTGAAGCA |
| TUR | 31,125,760 | 31,125,782 | GACTCACTTCCTCGCTTCAAGAC |
| TDF | 31,117,546 | 31,117,563 | GCAGCAAAGCCGCAACTC |
| TDP | 31,117,521 | 31,117,544 | AACCCAAGCCTTCTGGTTGGCAGA |
| TDR | 31,117,501 | 31,117,520 | CAGGACCGACAAGCTGTAAC |
PCR Primers
| Primer | Sequence |
| SU | AGCAACGTCCGGAGCCAG |
| SD | TGCTTCACCCATCATGCATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 63 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
