11881 Alb Status: ES cell colonies screened / QC no positives
| Gene | Alb |
| Chromosome | 5 |
| Deletion Start | 90,460,938 |
| Deletion End | 90,475,317 |
| Deletion Size | 14,380 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,461,566 | 90,461,589 | GCCACCTTCAGATTTTCCTGTAAC |
| TUP | 90,461,592 | 90,461,614 | TCGGGAACTGGCATCTTCAGGGA |
| TUR | 90,461,615 | 90,461,639 | TCTTCTCTTCACTGACCTAAGCTAC |
| TDF | 90,474,523 | 90,474,545 | GGGTAAATGTCTCCTTGCTCTTC |
| TDP | 90,474,546 | 90,474,569 | TTGCTTTCTGTCCTACTGCAGGGC |
| TDR | 90,474,597 | 90,474,617 | AGTTGCTCCGCTGTAGCCTTG |
PCR Primers
| Primer | Sequence |
| SU | TGAATGGACAAAGTCTTGTGC |
| SD | TAACTCCTTTGCTCTGGTTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
