11882 Lfng Status: ES cell colonies screened / QC no positives
| Gene | Lfng |
| Chromosome | 5 |
| Deletion Start | 140,607,419 |
| Deletion End | 140,614,476 |
| Deletion Size | 7,058 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 140,608,008 | 140,608,031 | CACTTTGCCTTACTACCCTGGAAG |
| TUP | 140,608,033 | 140,608,055 | TTCCTTCCGTGTTGCCCAGGGTC |
| TUR | 140,608,081 | 140,608,100 | TAAGCTGGAGGCGACTCATC |
| TDF | 140,614,153 | 140,614,172 | GCCACCTCTCCAGCATCATC |
| TDP | 140,614,176 | 140,614,197 | TTGCTCCCAATGCAGGTGACCC |
| TDR | 140,614,217 | 140,614,235 | CACTGCGTTCCGCTTGTTC |
PCR Primers
| Primer | Sequence |
| SU | CGTGGGAAGGAGAGATCTC |
| SD | CTGCAGAGATCCAAACACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
