11894 Rpl6 Status: Cancelled
| Gene | Rpl6 |
| Chromosome | 5 |
| Deletion Start | 121,205,409 |
| Deletion End | 121,208,952 |
| Deletion Size | 3,544 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,206,324 | 121,206,347 | AACTCCCACTCCCAAGGATAACTC |
| TUP | 121,206,350 | 121,206,375 | TGGCACATGTACTCATGGGCATACCC |
| TUR | 121,206,379 | 121,206,399 | GGAATGCGGTTTGCATGTGTC |
| TDF | 121,208,660 | 121,208,680 | AGTGTGGCAGTCTGAGTTGAG |
| TDP | 121,208,682 | 121,208,711 | TGGTTGACTTAGAGTAGGCAAGCTCCAAGG |
| TDR | 121,208,736 | 121,208,756 | GAATCAGGACGGCAGGAACAG |
PCR Primers
| Primer | Sequence |
| SU | TAGGCTGCTGGCACTGAAGT |
| SD | CCCTGAGAAAGACTTGAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
