11896 Ccdc28b Status: Cancelled
| Gene | Ccdc28b |
| Chromosome | 4 |
| Deletion Start | 129,622,716 |
| Deletion End | 129,620,415 |
| Deletion Size | 2,302 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 129,622,641 | 129,622,658 | TCCTTGGCTCTGGGACTC |
| TUP | 129,622,615 | 129,622,638 | TCATTTGCCATCACCCAAGCAGCG |
| TUR | 129,622,589 | 129,622,611 | TCCCTACTCACCTCTTGAACTTG |
| TDF | 129,621,272 | 129,621,292 | GCTCCTTCCCTTAACCTATCC |
| TDP | 129,621,251 | 129,621,271 | TGCTGCTCAGGACAGGCCCAG |
| TDR | 129,621,219 | 129,621,239 | CCCACTCACCAACACAAACTG |
PCR Primers
| Primer | Sequence |
| SU | CTCTGGGCTGTAGTGACAGG |
| SD | CTCACTATGTAGATCTGGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
