11898 Slx1b Status: Cancelled
| Gene | Slx1b |
| Chromosome | 7 |
| Deletion Start | 126,694,801 |
| Deletion End | 126,690,813 |
| Deletion Size | 3,989 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,695,509 | 126,695,529 | CCGCGTTTACGTTGGATTCAC |
| TUP | 126,695,486 | 126,695,508 | TGTCAATCCTGCTCGTCGGGTCC |
| TUR | 126,695,446 | 126,695,466 | CGCCATGCTCCACCTTTCTTG |
| TDF | 126,692,558 | 126,692,575 | TTCCACTTGCGCGTTCTG |
| TDP | 126,692,533 | 126,692,556 | CGCACATGCTTCGAGTTCCTCCGT |
| TDR | 126,692,481 | 126,692,499 | TCATGGCGGAAGTCAGGTC |
PCR Primers
| Primer | Sequence |
| SU | GTTCCGCTTGCACACCCTC |
| SD | CCATGGGAAAGCAACTGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
