11900   2210407C18Rik     Status: ES cell colonies screened / QC no positives

Gene2210407C18Rik
Chromosome11
Deletion Start
Deletion End
Deletion Size1
Genome Build37
MGI IDMGI:1925604
NCBI Gene ID78354
BAC ID80O13
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF58,612,49458,612,511AAGCCAACCCTCGCTACC
TUP58,612,46758,612,492TGAGCATCCTCCAATTCATCCTTGCC
TUR58,612,43458,612,453TCCACCCAGCTCCTAGTCTC
TDF58,609,01858,609,038AGGGAAAGGGTCTTTGTGGAG
TDP58,608,98458,609,012TTGAGATGAACAGGGACAATGGGATGTCA
TDR58,608,94458,608,965CCCTGCTATGGATGGCTTCAAC

PCR Primers

PrimerSequence
SUGGATCTAGTGCCTTCCAGTC
SDAGCTTGCCTCTTTATTCTTGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR78 bp++-
TDFTDR95 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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