11901 G6pc Status: Cancelled
| Gene | G6pc |
| Chromosome | 11 |
| Deletion Start | 101,367,800 |
| Deletion End | 101,376,823 |
| Deletion Size | 9,024 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,367,910 | 101,367,931 | CCTGAGGAACGCCTTCTATGTC |
| TUP | 101,367,942 | 101,367,971 | TCTGGTTCCATCTTAAAGAGACTGTGGGCA |
| TUR | 101,367,973 | 101,367,991 | TGCCACCCAGAGGAGATTG |
| TDF | 101,376,568 | 101,376,587 | GAAGAGCTGCAAGGGAGAAC |
| TDP | 101,376,588 | 101,376,610 | TCAGCAAGTTGCTCCCATTCCGC |
| TDR | 101,376,619 | 101,376,640 | GAGGACCAAGGAAGCCACAATG |
PCR Primers
| Primer | Sequence |
| SU | TAGCACTGTCAAGCAGTGTG |
| SD | CGCAAGAGCATTCTCAGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
