11904 Rps8 Status: ES cell colonies screened / QC no positives
| Gene | Rps8 |
| Chromosome | 4 |
| Deletion Start | 117,156,112 |
| Deletion End | 117,153,910 |
| Deletion Size | 2,203 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 117,155,834 | 117,155,851 | GCACGCGGATGATGACAC |
| TUP | 117,155,811 | 117,155,832 | TGGGTATGCTGCACACTCCCGA |
| TUR | 117,155,773 | 117,155,793 | ACGCTCAGCTCTCCAAGGTTG |
| TDF | 117,154,038 | 117,154,057 | GCCAGCGTGGACTTCTACAG |
| TDP | 117,154,012 | 117,154,036 | TGGCGTGGAGTGTCTTGTCCTTTAC |
| TDR | 117,153,968 | 117,153,987 | TGGTCGTGAGGCAATACAGG |
PCR Primers
| Primer | Sequence |
| SU | ATAGCTCAGCTTAAGGAGCC |
| SD | CAAGCATGATTTACACACACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
