11907 Ptgfr Status: Cancelled
| Gene | Ptgfr |
| Chromosome | 3 |
| Deletion Start | 151,834,555 |
| Deletion End | 151,800,272 |
| Deletion Size | 34,284 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 151,835,230 | 151,835,252 | TTTCCTCGGACTCTTAGCTCTTG |
| TUP | 151,835,202 | 151,835,226 | TTTCCTTCTCGTGCAATGCCGTCAC |
| TUR | 151,835,164 | 151,835,184 | GCTGGCTTCTGAACTTCACTC |
| TDF | 151,801,780 | 151,801,801 | GAAGGCTGTCCTTAGGAACCTG |
| TDP | 151,801,753 | 151,801,776 | AAACTTGCCAGTCGTTGCTGTGGA |
| TDR | 151,801,725 | 151,801,747 | CCCAGATATGCAAGCTGATGATG |
PCR Primers
| Primer | Sequence |
| SU | GAGACAACCAGAACAGATGC |
| SD | TCCAAGAACGTAAGCCACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
