11908 Mrps7 Status: Cancelled
| Gene | Mrps7 |
| Chromosome | 11 |
| Deletion Start | 115,604,164 |
| Deletion End | 115,607,048 |
| Deletion Size | 2,885 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,604,758 | 115,604,776 | TTCCGGGAGCCCTTGATTG |
| TUP | 115,604,779 | 115,604,805 | AAGGAATATTACCGCAAGCCGGTGGCG |
| TUR | 115,604,815 | 115,604,837 | TCCTGGTCATACTTCTCCTCTTC |
| TDF | 115,606,339 | 115,606,359 | CTGTGCTTACCTGCCATTGTC |
| TDP | 115,606,360 | 115,606,383 | TGGGCTAGTCCTGGACCCATGACC |
| TDR | 115,606,395 | 115,606,416 | AGGGACCACTACTTCAGTTCAG |
PCR Primers
| Primer | Sequence |
| SU | CGAGGATGTGTCTTCGCTTC |
| SD | TGGAGAATCAAAGACTCACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
