11913 Cyb5r1 Status: Cancelled
| Gene | Cyb5r1 |
| Chromosome | 1 |
| Deletion Start | 134,406,042 |
| Deletion End | 134,411,104 |
| Deletion Size | 5,063 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,406,129 | 134,406,149 | GTGCCATACCCACCTTGGATG |
| TUP | 134,406,151 | 134,406,171 | TGGAGTCTCGCTGCACGAGCT |
| TUR | 134,406,179 | 134,406,199 | AGCAAGCGGAGCAGTGAACTC |
| TDF | 134,410,249 | 134,410,270 | GAAGCAGGAGAAGATGGAGGTG |
| TDP | 134,410,271 | 134,410,295 | TCTCATGCTGACCATAGGCCCTTGA |
| TDR | 134,410,339 | 134,410,357 | TGCTGAGGACCAGGAAGTG |
PCR Primers
| Primer | Sequence |
| SU | AATTAGCCTGGCTCCATGTC |
| SD | CTCCTTGAGCCTCATCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
