11916 Renbp Status: ES cell colonies screened / QC no positives
| Gene | Renbp |
| Chromosome | X |
| Deletion Start | 73,930,511 |
| Deletion End | 73,922,057 |
| Deletion Size | 8,455 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,929,802 | 73,929,821 | GCACCACCTGGCAAGAAATG |
| TUP | 73,929,776 | 73,929,797 | TTTGTGCTGACTCGGGATGGCC |
| TUR | 73,929,755 | 73,929,772 | TCCGCTGCACCTTCACTG |
| TDF | 73,922,367 | 73,922,387 | AAGGTGGCCCTCACCATCAAG |
| TDP | 73,922,335 | 73,922,361 | TCCTTTTAAAGGTGAGCGAGGAACGGG |
| TDR | 73,922,309 | 73,922,328 | GGTGCCACAAACCCCTACAG |
PCR Primers
| Primer | Sequence |
| SU | TCTGAAGAGGATCAGGTGGG |
| SD | CAGGTCCCTCGATAACATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
