| Gene | Ogg1 |
| Chromosome | 6 |
| Deletion Start | 113,327,229 |
| Deletion End | 113,333,967 |
| Deletion Size | 6,739 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,327,261 | 113,327,281 | ACATCGCACCCTAAGCTCCAG |
| TUP | 113,327,287 | 113,327,310 | CCCTCTGGGCCTCTATCCCGTGTC |
| TUR | 113,327,352 | 113,327,370 | TCACTCACCGGAAGGACTG |
| TDF | 113,333,394 | 113,333,417 | TGGATGTCCATGTATGGCAGATTG |
| TDP | 113,333,418 | 113,333,439 | CCCATCGTGACTACGGCTGGCA |
| TDR | 113,333,479 | 113,333,502 | CACTCCTCACCCAGTTCTTTGTTG |
PCR Primers
| Primer | Sequence |
| SU | TTCCAAACCTCGAGGGCTG |
| SD | CTCACTCTTCACTGCGTTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11923A-A7 | VGB6 | C57BL/6NTac | |
| 11923A-G7 | VGB6 | C57BL/6NTac | |
| 11923A-H2 | VGB6 | C57BL/6NTac | |
| 11923A-H3 | VGB6 | C57BL/6NTac | |
| 11923A-H9 | VGB6 | C57BL/6NTac | |
