11926 Dexi Status: Cancelled
| Gene | Dexi |
| Chromosome | 16 |
| Deletion Start | 10,530,880 |
| Deletion End | 10,530,576 |
| Deletion Size | 305 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,542,607 | 10,542,625 | ACCGCTGCTGCCCTCTATG |
| TUP | 10,542,582 | 10,542,605 | TCTACGTGGGCCTGTTCTTCGTCA |
| TUR | 10,542,553 | 10,542,576 | AAGGAAGGCGTAGTAAAGGATCAG |
| TDF | 10,542,502 | 10,542,519 | TTCCTGCCCGAGGACTTG |
| TDP | 10,542,471 | 10,542,494 | CGCTGGTGGACCTCGGCGTACTTT |
| TDR | 10,542,441 | 10,542,460 | CGGCATCGTAAAGGCCAGAG |
PCR Primers
| Primer | Sequence |
| SU | TACAAAGCGAGTCGCTGGC |
| SD | CACTACTATTCCTAGCATCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
