11929 Pxmp3 Status: Cancelled
| Gene | Pxmp3 |
| Chromosome | 3 |
| Deletion Start | 5,575,910 |
| Deletion End | 5,574,995 |
| Deletion Size | 916 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,561,708 | 5,561,732 | GAGAATATGCAGAGTGCAAACAGAG |
| TUP | 5,561,681 | 5,561,707 | TGCTGAGGATAAGTCAGTTGGATGCAC |
| TUR | 5,561,656 | 5,561,675 | TGCTCCAGGGCCTTATTCAG |
| TDF | 5,561,073 | 5,561,096 | GCCAAATTGTCTTCCTGGTGTATC |
| TDP | 5,561,047 | 5,561,072 | CCTCTTACTGGTGCTGCTGGTCATGA |
| TDR | 5,561,026 | 5,561,046 | CCACTGCTGCCTAGTGTACTG |
PCR Primers
| Primer | Sequence |
| SU | ATAGGGTCAAGATATAAAGGAG |
| SD | TCCAGTGATTAGAACTGAGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
