11932 Polr2i Status: Cancelled
| Gene | Polr2i |
| Chromosome | 7 |
| Deletion Start | 30,232,349 |
| Deletion End | 30,233,436 |
| Deletion Size | 1,088 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,232,750 | 30,232,770 | TGCCGGAATTGCGATTACCAG |
| TUP | 30,232,771 | 30,232,794 | CAGGAAGCCGACAACAGCTGCATC |
| TUR | 30,232,813 | 30,232,832 | GCCACTCACTCCACTTCGTG |
| TDF | 30,233,228 | 30,233,248 | TTTGGTCCTCACACTATCCTC |
| TDP | 30,233,249 | 30,233,271 | TGGGTCCAGACCCTGACTGAACG |
| TDR | 30,233,274 | 30,233,294 | GCATGGCGTCCTAGCAATAAG |
PCR Primers
| Primer | Sequence |
| SU | GGATTCTCTCGTTCACGCTC |
| SD | CCTCCCTCTCTCACAAGACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
