11933 Lyrm2 Status: Cancelled
| Gene | Lyrm2 |
| Chromosome | 4 |
| Deletion Start | 32,800,294 |
| Deletion End | 32,801,236 |
| Deletion Size | 943 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,800,665 | 32,800,687 | GGCAAGTTCCAAGTGATTCTGAC |
| TUP | 32,800,695 | 32,800,716 | ACCTTCAGGATTGGGCCAGGGA |
| TUR | 32,800,745 | 32,800,764 | GGTCTTGCTCTCACCTCTTC |
| TDF | 32,800,777 | 32,800,795 | CGGCTCCTTTGTCCCATTG |
| TDP | 32,800,799 | 32,800,827 | CACTGAGCTTGTGAAGTTTAGCGAACACC |
| TDR | 32,800,843 | 32,800,862 | TGGGCAGTGAGCAAATTCTC |
PCR Primers
| Primer | Sequence |
| SU | AAGCTTCCAGGATGCAAACG |
| SD | TCATCTTGACATTAGTTCACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
