11934 Glrx Status: ES cell colonies screened / QC no positives
| Gene | Glrx |
| Chromosome | 13 |
| Deletion Start | 75,839,972 |
| Deletion End | 75,847,301 |
| Deletion Size | 7,330 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,840,324 | 75,840,342 | GGGACTCAAGGGAAGACAC |
| TUP | 75,840,345 | 75,840,370 | CTCAAATGAGTGGGCAGCAGGACTGC |
| TUR | 75,840,395 | 75,840,416 | CCACTATCCACACACCAACCAG |
| TDF | 75,846,377 | 75,846,401 | CCCAGGTTTGGTATAAGGTCTCTTC |
| TDP | 75,846,402 | 75,846,424 | TCAGGTCTGGCTCCAACAGCAGG |
| TDR | 75,846,425 | 75,846,446 | CACTAGGCTGCGCTTCAGATTC |
PCR Primers
| Primer | Sequence |
| SU | AGCCTGTTATAGCTTACACAG |
| SD | TGTAGTGCAATACATCAAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
