11936 Irf6 Status: Cancelled
| Gene | Irf6 |
| Chromosome | 1 |
| Deletion Start | 193,157,222 |
| Deletion End | 193,169,517 |
| Deletion Size | 12,296 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 193,158,133 | 193,158,157 | GTGGTTATAGAAGGGTTGCATTGTG |
| TUP | 193,158,166 | 193,158,191 | TTCAGGAGCTGCCTCTCCACTTTAGA |
| TUR | 193,158,196 | 193,158,216 | TCCTGAGATGGAAGGGAGAAG |
| TDF | 193,168,649 | 193,168,671 | TCCATCACAGCAACTCTTCTTTC |
| TDP | 193,168,681 | 193,168,708 | TTTACCCATTGGTCTGGGATGAGTCCTG |
| TDR | 193,168,709 | 193,168,732 | CACATAGGTCAGTGGATGCTCAAC |
PCR Primers
| Primer | Sequence |
| SU | GAGCAGTTGCCTTAGCCTTG |
| SD | AACACAATGCAGAAGGGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
