11938 Plekhf2 Status: Cancelled
| Gene | Plekhf2 |
| Chromosome | 4 |
| Deletion Start | 11,005,665 |
| Deletion End | 11,004,896 |
| Deletion Size | 770 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,991,231 | 10,991,249 | TCCCTGGGCGAGTGCTTAT |
| TUP | 10,991,202 | 10,991,230 | TGGAGAAGGAGTGTTGACTAAGTTGTGCA |
| TUR | 10,991,181 | 10,991,201 | GCCTTGCTTTAGGCTTCTTTC |
| TDF | 10,990,839 | 10,990,863 | CGCTGTCAGAAAGCAAAATTCACAC |
| TDP | 10,990,816 | 10,990,838 | CAGTCAATCGGCGGCACCATTGC |
| TDR | 10,990,778 | 10,990,797 | TCAGAGCAGGGACCACAAAC |
PCR Primers
| Primer | Sequence |
| SU | GTTCTGATGCCTAGTGATTC |
| SD | TAAGGACAGTAGAAAGCCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
