11939 Krba1 Status: ES cell colonies screened / QC no positives
| Gene | Krba1 |
| Chromosome | 6 |
| Deletion Start | 48,402,980 |
| Deletion End | 48,416,394 |
| Deletion Size | 13,415 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,403,809 | 48,403,828 | CAGACCACAGGGCCTTTCAG |
| TUP | 48,403,833 | 48,403,861 | ACCTTAGAAAGAAAGGAGACAGGAGGAGC |
| TUR | 48,403,879 | 48,403,900 | GCTGGACTTAGGAGGGTTCTTG |
| TDF | 48,416,030 | 48,416,048 | GGGCAGTTTGGTGCCTCAG |
| TDP | 48,416,050 | 48,416,072 | TGCCTCCAGAGGCTTCCTTGGTA |
| TDR | 48,416,087 | 48,416,107 | TGCTGGGATGAACTACAGGTG |
PCR Primers
| Primer | Sequence |
| SU | ACCTACTTCTGCACACCAAG |
| SD | GCCAAGAAAGTCAGAGGTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
