Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

11940 Med28 Status: ES cell colonies screened / QC positives

Gene	Med28
Chromosome	5
Deletion Start	45,520,329
Deletion End	45,525,352
Deletion Size	5,024
Genome Build	38

MGI ID	MGI:1914249
NCBI Gene ID	66999
BAC ID	412B6
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 5

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	45,520,451	45,520,472	CACTTTGGTGGACGAGTTGGAG
TUP	45,520,474	45,520,500	CATCCTTCGAGGTAAGCCGAGATGTGG
TUR	45,520,503	45,520,520	TGAGGGCTGCACAGAGAC
TDF	45,525,245	45,525,266	GGACATCAATGTGCAGCACAAG
TDP	45,525,270	45,525,290	CCGGCTGACATGCCTCAGGGA
TDR	45,525,323	45,525,342	GCTTCAGAGGTGCAGGAATG

PCR Primers

Primer	Sequence
SU	GAAAAGACTCGGCTAAGGTC
SD	GATGAAGACACAGAATGTTGTC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	70 bp	+	+	-
TDF	TDR	98 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
11940A-A8	VGB6	C57BL/6NTac
11940A-B3	VGB6	C57BL/6NTac
11940A-F12	VGB6	C57BL/6NTac
11940A-C12	VGB6	C57BL/6NTac